👤 N Nemani

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2
Articles
2
Name variants
Also published as: Neeharika Nemani
articles

A Selective and Cell-Permeable Mitochondrial Calcium Uniporter (MCU) Inhibitor Preserves Mitochondrial Bioenergetics after Hypoxia/Reoxygenation Injury

Joshua J. Woods, Neeharika Nemani, Santhanam Shanmughapriya +10 more · 2019 · ACS Central Science · ACS Publications · added 2026-04-20
Mitochondrial Ca2+ (mCa2+) uptake mediated by the mitochondrial calcium uniporter (MCU) plays a critical role in signal transduction, bioenergetics, and cell death, an Show more
Mitochondrial Ca2+ (mCa2+) uptake mediated by the mitochondrial calcium uniporter (MCU) plays a critical role in signal transduction, bioenergetics, and cell death, and its dysregulation is linked to several human diseases. In this study, we report a new ruthenium complex Ru265 that is cell-permeable, minimally toxic, and highly potent with respect to MCU inhibition. Cells treated with Ru265 show inhibited MCU activity without any effect on cytosolic Ca2+ dynamics and mitochondrial membrane potential (ΔΨm). Dose-dependent studies reveal that Ru265 is more potent than the currently employed MCU inhibitor Ru360. Site-directed mutagenesis of Cys97 in the N-terminal domain of human MCU ablates the inhibitory activity of Ru265, suggesting that this matrix-residing domain is its target site. Additionally, Ru265 prevented hypoxia/reoxygenation injury and subsequent mitochondrial dysfunction, demonstrating that this new inhibitor is a valuable tool for studying the functional role of the MCU in intact biological models. Show less
no PDF DOI: 10.1021/acscentsci.8b00773
mitochondria

Structure, Activity Regulation, and Role of the Mitochondrial Calcium Uniporter in Health and Disease

J Mammucari, A Sreedhar, Y Zhao +345 more · 2017 · Frontiers in Oncology · Frontiers · added 2026-04-20
J Mammucari, A Sreedhar, Y Zhao, TN Seyfried, LM Shelton, R Rizzuto, D De Stefani, A Raffaello, C Mammucari, Y Kirichok, G Krapivinsky, DE Clapham, B Mertins, G Psakis, L-O Essen, E Rapizzi, P Pinton, G Szabadkai, MR Wieckowski, G Vandecasteele, G Baird, A Bononi, A Romagnoli, A Messina, V De Pinto, V Shoshan-Barmatz, S De, A Meir, M Colombini, S Naghdi, G Hajnóczky, S Reina, F Guarino, A Magrì, R Palty, WF Silverman, M Hershfinkel, T Caporale, SL Sensi, J Parnis, M Giacomello, I Drago, M Bortolozzi, M Scorzeto, A Gianelle, P Pizzo, G Csordás, P Várnai, T Golenár, S Roy, G Purkins, TG Schneider, F Fieni, S Bae Lee, YN Jan, E Teardo, I Szabò, JM Baughman, F Perocchi, HS Girgis, M Plovanich, CA Belcher-Timme, Y Sancak, VM Gohil, XR Bao, JE McCombs, AE Palmer, JD Martell, TJ Deerinck, TL Poulos, VK Mootha, GE Sosinsky, D Sabbadin, G Merli, A Picard, Y Lee, CK Min, TG Kim, HK Song, Y Lim, D Kim, K Oxenoid, Y Dong, C Cao, T Cui, AL Markhard, S Wang, X-C Su, JJ Chou, G Huang, AE Vercesi, R Docampo, J Prudent, N Popgeorgiev, B Bonneau, J Thibaut, R Gadet, J Lopez, RL Davis, S Xu, AD Chisholm, E Murphy, X Pan, T Nguyen, J Liu, KM Holmström, T Finkel, ME Dickinson, AM Flenniken, X Ji, L Teboul, MD Wong, JK White, C Liu, J Sun, Y Teng, ALL Markhard, T Kitami, E Kovacs-Bogdan, KJJ Kamer, NDD Udeshi, JC Liu, S Menazza, RJ Parks, MM Fergusson, A Jambhekar, RJJ Huber, H Vais, K Mallilankaraman, D-OD Mak, H Hoff, R Payne, JE Tanis, T Yamamoto, R Yamagoshi, K Harada, M Kawano, N Minami, Y Ido, M-F Tsai, CB Phillips, M Ranaghan, C-W Tsai, Y Wu, C Williams, T König, SE Tröder, K Bakka, A Korwitz, R Richter-Dennerlein, PA Lampe, P-C Pao, C Miller, P Doonan, C Cárdenas, HC Chandramoorthy, M Müller, R Miller, NE Hoffman, S Shamugapriya, X Zhang, S Rajan, EL Seifert, KJ Kamer, L Wang, X Yang, S Li, Z Wang, Y Liu, J Feng, CV Logan, JA Sharpe, DA Parry, S Torelli, A-M Childs, AN Antony, M Paillard, C Moffat, E Juskeviciute, J Correnti, B Bolon, M Patron, V Checchetto, D Vecellio Reane, M Mantoan, G Szanda, V Debattisti, A Bartok, PG M’Angale, BE Staveley, RL Bogorad, L Strittmatter, AA Li, C Petrungaro, KM Zimmermann, V Küttner, M Fischer, J Dengjel, I Bogeski, F Vallese, L Acquasaliente, G Butera, V De Filippis, PJ Doonan, KM Irrinki, D Tomar, Z Dong, S Shanmughapriya, DA Koch, T Thomas, V Paupe, EP Dassa, OZ Rendon, EA Shoubridge, JK Foskett, ND Udeshi, D Chaudhuri, DJ Artiga, SA Abiria, S Guo, JE Kolesar, J Qiu, Y-W Tan, AM Hagenston, M-A Martel, N Kneisel, PA Skehel, S Marchi, L Lupini, S Patergnani, A Rimessi, S Missiroli, M Bonora, L Pan, B-J Huang, X-E Ma, S-Y Wang, F Lv, Z Hong, KH Chen, A Dasgupta, F Potus, K Dunham-Snary, S Bonnet, MA Joiner, OM Koval, J Li, BJ He, C Allamargot, Z Gao, DE Johnson, A Hudmon, J O-Uchi, BS Jhun, S Hurst, X Liu, N Siddiqui, S Lynch, N Nemani, CT Madreiter-Sokolowski, C Klec, W Parichatikanond, S Stryeck, B Gottschalk, S Pulido, D Hanahan, RA Weinberg, DD Hall, FE Domann, DR Spitz, ME Anderson, S Tang, X Wang, Q Shen, C Yu, C Cai, MC Curry, AA Peters, PA Kenny, SJ Roberts-Thomson, GR Monteith, A Tosatto, R Sommaggio, C Kummerow, RB Bentham, TS Blacker, T Berecz, X Zhou, Y Ren, L Kong, G Cai, S Sun, W Song, J Long, Z-B Zhang, Z Liu, Y-H Xu, C-L Ge, MJ Yoon, AR Lee, SA Jeong, Y-S Kim, JY Kim, Y-J Kwon, C Wiel, H Lallet-Daher, D Gitenay, B Gras, B Le Calvé, A Augert, T Pozzan, TT Nguyen, TP Rasmussen, M-LA Joiner, B Chen, NR Wilson, ED Luczak, JQ Kwong, X Lu, RN Correll, JA Schwanekamp, RJ Vagnozzi, MA Sargent, TS Luongo, JP Lambert, A Yuan, P Gross, J Song, G Gherardi, I Zamparo, S Boncompagni, F Chemello, S Zampieri, V Romanello, L Barberi, L Pietrangelo, A Fusella, AI Tarasov, F Semplici, MA Ravier, EA Bellomo, TJ Pullen, P Gilon, L Gu, JL Larson-Casey, AB Carter, V Bezzerri, G Cabrini Show less
Mitochondrial Ca 2+ uptake plays a pivotal role both in cell energy balance and in cell fate determination. Studies on the role of mitochondrial Ca 2+ signaling in pathophysiology have been favored Show more
Mitochondrial Ca 2+ uptake plays a pivotal role both in cell energy balance and in cell fate determination. Studies on the role of mitochondrial Ca 2+ signaling in pathophysiology have been favored by the identification of the genes encoding the mitochondrial calcium uniporter (MCU) and its regulatory subunits. Thus, research carried on in the last years on one hand has determined the structure of the MCU complex and its regulation, on the other has uncovered the consequences of dysregulated mitochondrial Ca 2+ signaling in cell and tissue homeostasis. Whether mitochondrial Ca 2+ uptake can be exploited as a weapon to counteract cancer progression is debated. In this review, we summarize recent research on the molecular structure of the MCU, the regulatory mechanisms that control its activity and its relevance in pathophysiology, focusing in particular on its role in cancer progression. Show less
📄 PDF DOI: 10.3389/fonc.2017.00139
mitochondria review