Hydrogen peroxide (H2 O2 ) is an important reactive oxygen species that plays a major
role in redox signaling. Although H2 O2 is known to regulate gene expression and affect multiple
cellular processe Show more
Hydrogen peroxide (H2 O2 ) is an important reactive oxygen species that plays a major
role in redox signaling. Although H2 O2 is known to regulate gene expression and affect multiple
cellular processes, the characteristics and mechanisms of such transcriptional regulation remain to
be defined. In this study, we utilized transcriptome sequencing to determine the global changes of
mRNA and lncRNA transcripts induced by H2 O2 in human pancreatic normal epithelial (HPNE)
and pancreatic cancer (PANC-1) cells. Promoter analysis using PROMO and TRRUST revealed
that mRNAs and lncRNAs largely shared the same sets of transcription factors in response to ROS
stress. Interestingly, promoters of the upregulated genes were similar to those of the downregulated
transcripts, suggesting that the H2 O2 -responding promoters are conserved but they alone do not
determine the levels of transcriptional outputs. We also found that H2 O2 induced significant changes
in molecules involved in the pathways of RNA metabolism, processing, and transport. Detailed
analyses further revealed a significant difference between pancreatic cancer and noncancer cells in
their response to H2 O2 stress, especially in the transcription of genes involved in cell-cycle regulation
and DNA repair. Our study provides new insights into RNA transcriptional regulation upon ROS
stress in cancer and normal cells.
Transcription of mRNA and lncRNA.
Antioxidants 2022, 11, 495. https:// Show less
Berico P, Coin F · 2018 · Transcription · Taylor & Francis · added 2026-04-20
TFIIH is a 10-subunit complex involved in transcription and DNA repair. It contains several enzymatic activities including a ATP-dependent DNA translocase in XPB and a cyclin-dependent kinase in CDK7. Show more
TFIIH is a 10-subunit complex involved in transcription and DNA repair. It contains several enzymatic activities including a ATP-dependent DNA translocase in XPB and a cyclin-dependent kinase in CDK7. Recently the discovery of several XPB and CDK7 inhibitors with specific impact on the transcriptional addiction of many tumors pinpointed these activities as potential target in cancer chemotherapy. Unexpectedly a basal transcription factor involved in global mRNA expression now emerges a one of the most clinically promising Achilles heels of cancerous cells. These inhibitors also proved to be useful tools to unveil new functions of TFIIH in gene expression. Show less